8VYQ | pdb_00008vyq

Cryo-EM Structure of the BRAF V600K monomer

PDB DOI: https://doi.org/10.2210/pdb8VYQ/pdb
EM Map EMD-43675: EMDB EMDataResource

Classification: TRANSFERASE/INHIBITOR
Organism(s): Homo sapiens
Expression System: Homo sapiens
Mutation(s): Yes

Deposited: 2024-02-09 Released: 2025-05-28
Deposition Author(s): Lavoie, H., Lajoie, D., Jin, T., Decossas, M., Maisonneuve, P., Therrien, M.
Funding Organization(s): Canadian Institutes of Health Research (CIHR)

Experimental Data Snapshot

Method: ELECTRON MICROSCOPY
Resolution: 4.43 Å
Aggregation State: PARTICLE
Reconstruction Method: SINGLE PARTICLE

wwPDB Validation 3D Report Full Report

This is version 1.0 of the entry. See complete history.

Literature

BRAF oncogenic mutants evade autoinhibition through a common mechanism

Lavoie, H., Jin, T., Lajoie, D., Decossas, M., Gendron, P., Wang, B., Filandr, F., Sahmi, M., Hwa Jo, C., Weber, S., Arseneault, G., Tripathy, S., Beaulieu, P., Schuetz, D.A., Schriemer, D.C., Marinier, A., Rice, W.J., Maisonneuve, P., Therrien, M.

(2025) Science

DOI: https://doi.org/10.1126/science.adp2742

Macromolecule Content

Total Structure Weight: 97.83 kDa
Atom Count: 2,337
Modeled Residue Count: 470
Deposited Residue Count: 865
Unique protein chains: 2

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
14-3-3 protein zeta/delta	A, B	245	Homo sapiens	Mutation(s): 0
UniProt & NIH Common Fund Data Resources
Find proteins for P63104 (Homo sapiens) Explore P63104 Go to UniProtKB: P63104
PHAROS: P63104 GTEx: ENSG00000164924
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P63104
Sequence Annotations Expand
Reference Sequence

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 2
Molecule	Chains	Sequence Length	Organism	Details	Image
Serine/threonine-protein kinase B-raf	C	375	Homo sapiens	Mutation(s): 1 Gene Names: BRAF, BRAF1, RAFB1 EC: 2.7.11.1
UniProt & NIH Common Fund Data Resources
Find proteins for P15056 (Homo sapiens) Explore P15056 Go to UniProtKB: P15056
PHAROS: P15056 GTEx: ENSG00000157764
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P15056
Sequence Annotations Expand
Reference Sequence

Small Molecules

Modified Residues 1 Unique
ID	Chains	Type	Formula	2D Diagram	Parent
SEP Query on SEP	C	L-PEPTIDE LINKING	C₃ H₈ N O₆ P		SER

Experimental Data & Validation

Experimental Data

Method: ELECTRON MICROSCOPY
Resolution: 4.43 Å
Aggregation State: PARTICLE
Reconstruction Method: SINGLE PARTICLE

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2025-05-28

Deposition Author(s):

Maisonneuve, P.

Funding Organization	Location	Grant Number
Canadian Institutes of Health Research (CIHR)	Canada	FDN-388023

Revision History (Full details and data files)

Version 1.0: 2025-05-28
Type: Initial release

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.