Download MendeleyTRIP12 structures reveal HECT E3 formation of K29 linkages and branched ubiquitin chains
Maiwald, S.A., Schneider, L.A., Vollrath, R., Liwocha, J., Maletic, M.D., Swatek, K.N., Mulder, M.P.C., Schulman, B.A.To be published.
9GKN | pdb_00009gkn
Structure of HECT E3 TRIP12 forming K29-linked Ubiquitin chains
- PDB DOI: https://doi.org/10.2210/pdb9GKN/pdb
- EM Map EMD-51430: EMDB EMDataResource
- Classification: LIGASE
- Organism(s): Homo sapiens
- Expression System: Escherichia coli, Trichoplusia ni
- Mutation(s): Yes
- Deposited: 2024-08-25 Released: 2025-05-21
- Funding Organization(s): German Research Foundation (DFG), Boehringer Ingelheim Fonds (BIF)
Experimental Data Snapshot
- Method: ELECTRON MICROSCOPY
- Resolution: 3.40 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Ubiquitin | A [auth B] | 75 | Homo sapiens | Mutation(s): 0 Gene Names: UBC |  |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P0CG48 (Homo sapiens) Explore P0CG48 Go to UniProtKB: P0CG48 | |||||
PHAROS: P0CG48 GTEx: ENSG00000150991 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P0CG48 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Polyubiquitin-C | B [auth C] | 78 | Homo sapiens | Mutation(s): 1 Gene Names: UBC |  |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P0CG48 (Homo sapiens) Explore P0CG48 Go to UniProtKB: P0CG48 | |||||
PHAROS: P0CG48 GTEx: ENSG00000150991 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P0CG48 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 3 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Isoform 3 of E3 ubiquitin-protein ligase TRIP12 | C [auth A] | 1,565 | Homo sapiens | Mutation(s): 0 Gene Names: TRIP12, KIAA0045, ULF EC: 2.3.2.26 |  |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for Q14669 (Homo sapiens) Explore Q14669 Go to UniProtKB: Q14669 | |||||
PHAROS: Q14669 GTEx: ENSG00000153827 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q14669 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SY8 Query on SY8 | D [auth B] | 5-azanylpentan-2-one C5 H11 N O VTHKLCWOLNQWHB-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: ELECTRON MICROSCOPY
- Resolution: 3.40 Å
- Aggregation State: PARTICLE
- Reconstruction Method: SINGLE PARTICLE
| Task | Software Package | Version |
|---|---|---|
| MODEL REFINEMENT | PHENIX | 1.21.1_5286 |
Entry History & Funding Information
Deposition Data
- Released Date: 2025-05-21 Deposition Author(s): Maiwald, S.A., Schulman, B.A.
| Funding Organization | Location | Grant Number |
|---|---|---|
| German Research Foundation (DFG) | Germany | SFB1035 |
| Boehringer Ingelheim Fonds (BIF) | Germany | -- |
Revision History (Full details and data files)
- Version 1.0: 2025-05-21
Type: Initial release
