Download MendeleyCrystallographic structure of AcrB V612F with bound minocycline
Lazarova, M., Diederichs, K., Pos, K.M.To be published.
9FHC | pdb_00009fhc
Crystallographic structure of AcrB V612F with bound minocycline
- PDB DOI: https://doi.org/10.2210/pdb9FHC/pdb
- Classification: TRANSPORT PROTEIN
- Organism(s): Escherichia coli K-12, synthetic construct
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2024-05-27 Released: 2025-06-11
- Funding Organization(s): Swiss National Science Foundation, German Research Foundation (DFG)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 ?
- R-Value Free: 0.238 (Depositor), 0.238 (DCC)
- R-Value Work: 0.208 (Depositor), 0.208 (DCC)
Starting Model: experimental
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This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Multidrug efflux pump subunit AcrB | 1,057 | Escherichia coli K-12 | Mutation(s): 1 Gene Names: acrB, acrE, b0462, JW0451 |  | |
UniProt | |||||
Find proteins for P31224 (Escherichia coli (strain K12)) Explore P31224 Go to UniProtKB: P31224 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P31224 | ||||
Sequence AnnotationsExpand | |||||
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Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DARPIN | 169 | synthetic construct | Mutation(s): 0 |  | |
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| MIY (Subject of Investigation/LOI) Query on MIY | C [auth A] | (4S,4AS,5AR,12AS)-4,7-BIS(DIMETHYLAMINO)-3,10,12,12A-TETRAHYDROXY-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2-
CARBOXAMIDE C23 H27 N3 O7 DYKFCLLONBREIL-KVUCHLLUSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.20 ?
- R-Value Free: 0.238 (Depositor), 0.238 (DCC)
- R-Value Work: 0.208 (Depositor), 0.208 (DCC)
Diffraction Data: https://doi.org/10.5281/zenodo.11472085
Space Group: I 2 3
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 227.46 | ¦Á = 90 |
| b = 227.46 | ¦Â = 90 |
| c = 227.46 | ¦Ă = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| XDS | data reduction |
| XDS | data scaling |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2025-06-11 Deposition Author(s): Lazarova, M., Diederichs, K., Pos, K.M.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Swiss National Science Foundation | Switzerland | 3100A0_118402 |
| German Research Foundation (DFG) | Germany | SFB807-P18 |
| German Research Foundation (DFG) | Germany | SFB1507-P3 |
| German Research Foundation (DFG) | Germany | DFG-EXC115 |
Revision History (Full details and data files)
- Version 1.0: 2025-06-11
Type: Initial release
