Download MendeleyStructure, Function, and Application of Bacterial ABC Transporters
Fan, C.(2020) Ph D Thesis,california Institute Of Technology
7S7Z | pdb_00007s7z
Crystal structure of iAChSnFR Acetylcholine Sensor precursor binding protein with choline bound
- PDB DOI: https://doi.org/10.2210/pdb7S7Z/pdb
- Classification: FLUORESCENT PROTEIN
- Organism(s): Thermoanaerobacter sp. X513
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): No
- Deposited: 2021-09-17 Released: 2021-10-13
- Funding Organization(s): Howard Hughes Medical Institute (HHMI), National Institutes of Health/National Institute on Drug Abuse (NIH/NIDA), Tobacco-Related Disease Research Program (TRDRP)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.55 ?
- R-Value Free: 0.197 (Depositor), 0.200 (DCC)
- R-Value Work: 0.155 (Depositor), 0.160 (DCC)
- R-Value Observed: 0.157 (Depositor)
Starting Model: experimental
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This is version 1.3 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| iAchSnFR Fluorescent Acetylcholine Sensor precursor binding protein | 293 | Thermoanaerobacter sp. X513 | Mutation(s): 0 |  | |
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| NHE Query on NHE | C [auth A] | 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID C8 H17 N O3 S MKWKNSIESPFAQN-UHFFFAOYSA-N |  | ||
| CHT (Subject of Investigation/LOI) Query on CHT | B [auth A] | CHOLINE ION C5 H14 N O OEYIOHPDSNJKLS-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.55 ?
- R-Value Free: 0.197 (Depositor), 0.200 (DCC)
- R-Value Work: 0.155 (Depositor), 0.160 (DCC)
- R-Value Observed: 0.157 (Depositor)
Space Group: P 21 21 21
Unit Cell:
| Length ( ? ) | Angle ( ? ) |
|---|---|
| a = 46.561 | ¦Á = 90 |
| b = 65.666 | ¦Â = 90 |
| c = 96.428 | ¦Ă = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| PHASER | phasing |
| Aimless | data scaling |
| XDS | data reduction |
| Blu-Ice | data collection |
Entry History & Funding Information
Deposition Data
- Released Date: 2021-10-13 Deposition Author(s): Fan, C., Borden, P.M., Looger, L.L., Lester, H.A., Rees, D.C.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Howard Hughes Medical Institute (HHMI) | United States | -- |
| National Institutes of Health/National Institute on Drug Abuse (NIH/NIDA) | United States | DA037161 |
| National Institutes of Health/National Institute on Drug Abuse (NIH/NIDA) | United States | DA043829 |
| National Institutes of Health/National Institute on Drug Abuse (NIH/NIDA) | United States | GM123582 |
| Tobacco-Related Disease Research Program (TRDRP) | United States | 23XT-0007 |
| Tobacco-Related Disease Research Program (TRDRP) | United States | 27IP-0057 |
Revision History (Full details and data files)
- Version 1.0: 2021-10-13
Type: Initial release - Version 1.1: 2021-11-03
Changes: Structure summary - Version 1.2: 2022-04-27
Changes: Database references - Version 1.3: 2023-10-18
Changes: Data collection, Refinement description
